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The main differences between prokaryotic and eukaryotic cells are that prokaryotic cells have no nucleus, while eukaryotic cells have a well-defined nucleus. Prokaryotic cells have no membrane-bound organelles, while eukaryotic cells have various organelles such as mitochondria, chloroplasts, endoplasmic reticulum, etc. Prokaryotic cells are smaller (0.1-5 μm) and unicellular, while eukaryotic cells are larger (10-100 μm) and mostly multicellular.