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Acid-fast stain The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses. How the Test is Performed Depending on where the infection may be in your body, your healthRead more
Acid-fast stain
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.
How the Test is Performed
Depending on where the infection may be in your body, your health care provider will collect one of these samples:
Urine
Stool
Sputum
Bone marrow
Tissue
The sample is then sent to a laboratory. Some of the sample is placed on a glass slide, stained, and heated. The cells in the sample hold onto the dye. The slide is then washed with an acid solution and a different stain is applied.
Bacteria that hold onto the first dye are considered “acid-fast” because they resist the acid wash. These types of bacteria are associated with TB and other infections.
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