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An autoclave is a device that sterilizes materials by generating steam under pressure. The principle of autoclave is moist heat sterilization, which destroys all microorganisms, including heat-resistant endospores, by heating the contents above the boiling point of water. The high temperature and pressure ensure the killing of microorganisms like viruses, fungi, bacteria, and heat-resistant spores. The high temperature and moist heat coagulate and denature microbial proteins and enzymes.