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Malaria parasites can be identified by examining under the microscope a drop of the patient’s blood, spread out as a “blood smear” on a microscope slide. Prior to the examination, the specimen is stained (most often with the Giemsa stain) to give the parasites a distinctive appearance. This techniquRead more
Malaria parasites can be identified by examining under the microscope a drop of the patient’s blood, spread out as a “blood smear” on a microscope slide. Prior to the examination, the specimen is stained (most often with the Giemsa stain) to give the parasites a distinctive appearance. This technique remains the gold standard for laboratory confirmation of malaria. However, it depends on the quality of the reagents, of the microscope, and on the experience of the laboratorian
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Medical laboratory diagnosis: The primary objective in laboratory diagnosis is to identify whether the diagnosed S. aureus isolate is methicillin-resistant. Since MRSA emerged as a problematic pathogen, a systematic diagnostic approach is necessary for early diagnosis so that treatment with appropriRead more
Medical laboratory diagnosis:
See lessThe primary objective in laboratory diagnosis is to identify whether the diagnosed S. aureus isolate is methicillin-resistant. Since MRSA emerged as a problematic pathogen, a systematic diagnostic approach is necessary for early diagnosis so that treatment with appropriate antibiotics can be initiated as early as possible. For species identification, slide and tube coagulase tests, latex agglutination tests, and PCR-based tests are used. For detection of MRSA, determination of minimum inhibitory concentration (MIC) of methicillin or oxacillin or cefoxitin using broth micro-dilution method, cefoxitin disk screen, oxacillin agar screen and latex agglutination test for PBP2a and molecular methods for detection of mecA are employed.